|
Three hundred and one cellulolytic bacterial were isolated from the 148 screening sources such as decomposed wood, soil, compost and leaf mold. Among them, strain KL-6 was found to have the highest of cellulase activity, and identified as species belonged to the genus Cellularnonas. Strain KL-6 was decompose up to 90% of the filter paper (whatman No. 1) substrate within 50 hours, and showed the colony halo formation (11 ㎝). The activities of CMCase (67 unit/㎖), FPase (70 unit/㎖) and β-glucosidase (0.68 unit/㎖) were obtained when this strain was cultured for 50 hrs at 30℃. Glucose was not found in detectable amounts at the FP medium. The optimum composition of nutrient medium for the cell growth by strain KL-6 was sucrose 0.5%, yeast extract 0.1%, (NH₄)₂HPO₄ 0.1%, K₂HPO₄ 0.1%, MgSO₄·7H₂O 0.01%, CaCl₂ 0.01%, NaCl 0.6%, CaCO₃ 0.1% and the optimum pH and temperature were 7.0 and 30℃, respectively.
|